FIELD: medicine.
SUBSTANCE: invention relates to molecular oncology. A test system is presented. The test system contains reagents for obtaining cDNA on a total RNA matrix using a reverse transcription reaction combined with polyadenylation, amplification of the cDNA library in real time. The test system contains highly specific primers for hsa-miR-7-5p, hsa-miR-26a-5p and hsa-miR-143-3p. It provides primary data on the relative expression of micro-RNA in blood plasma for the calculation of prognostic coefficients KmiR-26a and KmiR-143, which make it possible to assess the likelihood of the course of the disease with or without the development of remote/regional metastases. The test system has a high sensitivity and specificity, its use is possible with blood plasma.
EFFECT: test system provides a minimally invasive assessment of the likelihood of metastasis in patients with colorectal cancer, which makes it possible to personalize treatment tactics.
1 cl, 1 tbl
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