FIELD: molecular biology.
SUBSTANCE: described is a method for simultaneous quantitative determination of at least two microRNAs in venous blood plasma. Method includes extraction of RNA, conducting reverse transcription with primers and subsequent polymerase chain reaction in "real time" using selected primers and fluorescent-labelled TaqMan probes, as well as multiplex mode of detection of one of microRNA together with miR-39, taken as a marker of efficiency of RNA extraction.
EFFECT: possibility to perform all stages of analysis in one analytical series.
1 cl, 6 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| OLIGONUCLEOTIDES FOR DIAGNOSING EPILEPSY BY QUANTITATIVE PCR | 2023 |
|
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| METHOD OF PRODUCING MOUSE MODEL FOR STUDYING DUCHENNE MUSCULAR DYSTROPHY AND VERSIONS OF ITS THERAPY | 2023 |
|
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| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| PANEL OF PRIMERS FOR WHOLE GENOME SEQUENCING OF HUMAN PARAINFLUENZA VIRUSES TYPE 1 AND 2 | 2024 |
|
RU2833687C1 |
| METHOD FOR QUANTITATIVE ASSESSMENT OF T AND B-LYMPHOCYTES BY CONTENT OF EXCISION RINGS TREC AND KREC IN DNA SAMPLES USING DIGITAL POLYMERASE CHAIN REACTION | 2023 |
|
RU2822204C1 |
| METHOD FOR DETECTING MAMMARENAVIRUS GUANARITOENSE VIRUS BY DETECTR WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2835994C1 |
| METHOD FOR DETECTING RECOMBINANT FORMS OF HEPATITIS C VIRUS AND HEPATITIS C VIRUS DRUG RESISTANCE MUTATIONS TO DIRECT ANTIVIRAL DRUGS BY POLYMERASE CHAIN REACTION WITH SUBSEQUENT SEQUENCING | 2023 |
|
RU2824565C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING RNA OF YELLOW FEVER VIRUS BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
|
RU2824209C1 |
| METHOD FOR MULTIPLEX IDENTIFICATION OF 52 GENETIC MARKERS OF ECONOMICALLY VALUABLE FLAX TRAITS | 2023 |
|
RU2826718C1 |
