FIELD: molecular biology.
SUBSTANCE: described is a method for simultaneous quantitative determination of at least two microRNAs in venous blood plasma. Method includes extraction of RNA, conducting reverse transcription with primers and subsequent polymerase chain reaction in "real time" using selected primers and fluorescent-labelled TaqMan probes, as well as multiplex mode of detection of one of microRNA together with miR-39, taken as a marker of efficiency of RNA extraction.
EFFECT: possibility to perform all stages of analysis in one analytical series.
1 cl, 6 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| OLIGONUCLEOTIDES FOR DIAGNOSING EPILEPSY BY QUANTITATIVE PCR | 2023 |
|
RU2815113C1 |
| RECOMBINANT PLASMID DNA CODING microRNA hsa-miR-145-5p, AND ITS USE AS POSITIVE CONTROL IN REAL-TIME PCR FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT | 2025 |
|
RU2837874C1 |
| RECOMBINANT PLASMID DNA CODING microRNA hsa-miR-143-5p, AND ITS USE AS POSITIVE CONTROL IN REAL-TIME PCR FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT | 2025 |
|
RU2837872C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT BASED ON BIOMOLECULAR MARKER microRNA hsa-miR-143-5p BY QUANTITATIVE PCR AND USE THEREOF | 2025 |
|
RU2838258C1 |
| METHOD FOR PREDICTION OF RECURRENT MISCARRIAGE BY MICRORNA GENE EXPRESSION IN ENDOMETRIAL BIOPSY MATERIAL | 2025 |
|
RU2837879C1 |
| RECOMBINANT PLASMID DNA CODING microRNA hsa-miR-145-3p, AND ITS USE AS POSITIVE CONTROL IN REAL-TIME PCR FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT | 2025 |
|
RU2837873C1 |
| RECOMBINANT PLASMID DNA CODING microRNA hsa-miR-143-3p, AND ITS USE AS POSITIVE CONTROL IN REAL-TIME PCR FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT | 2025 |
|
RU2837882C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT BASED ON BIOMOLECULAR MARKER microRNA hsa-miR-143-3p BY QUANTITATIVE PCR AND USE THEREOF | 2025 |
|
RU2838257C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS FOR DIAGNOSING CARDIOVASCULAR DISEASES AND DETECTING RISK OF THEIR DEVELOPMENT BASED ON BIOMOLECULAR MARKER microRNA hsa-miR-145-3p BY QUANTITATIVE PCR AND USE THEREOF | 2025 |
|
RU2838260C1 |
| SET OF OLIGONUCLEOTIDES AND METHOD FOR ENRICHMENT OF GENOMIC CDNA OF VIRUS OF MURINE CONGO HAEMORRHAGIC FEVER GENOTYPE EUROPA-1 (V) BY POLYMERASE CHAIN REACTION | 2023 |
|
RU2839313C1 |
