METHOD FOR QUANTITATIVE DETERMINATION OF N-NITROSAMINES: N-DIMETHYLNITROSAMINE, N-METHYLETHYLNITROSAMINE, N-DIETHYLNITROSAMINE, N-DIBUTYLNITROSAMINE, N-DIPROPYLNITROSOAMINE, N-PIPERIDINE NITROSAMINE, N-PYRROLIDINE NITROSAMINE, N-MORPHOLIN NITROSAMINE, N-DIPHENYL NITROSAMINE, IN SAMPLES OF SMOKED MEAT PRODUCTS USING A METHOD OF CHROMATOGRAPHY-MASS SPECTROMETRY Russian patent published in 2018 - IPC G01N33/12 

FIELD: medicine.

SUBSTANCE: invention relates to medical toxicology studies. Method for quantitative determination of N-nitrosamines comprises sample preparation, solid-phase extraction (TPHE), and determination of specific nitrosamines according to a calibration chart, during sample preparation 200 ml of distilled water preliminary heated up to +55 °C are added to 20 g of the sample of crushed smoked meat products, the sample is maintained for 30 minutes and further filtrated, 1.5 g of potassium hydroxide are added to the filtrate, the resultant mixture of filtrate and potassium hydroxide is subjected to distillation by water vapor overheated to t_{steam generator}=100±5 °C to obtain 70 cm³ of distillate, said distillate is subjected to TPHE, the resultant eluates are analyzed by chromatography-mass spectrometry with selection of nine N-nitrosamines.

EFFECT: method for quantitative determination of N-nitrosamines is proposed.

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