FIELD: biotechnology.
SUBSTANCE: invention relates to methods for the production of recombinant protein and its isolation (options). The method includes subsequent placement of recombinant mammalian cells to several cultural media for obtainment of the first, the second, and the third cell cultures, as well as for obtainment of a producer cell culture, while a perfusion bioreactor, a production bioreactor, and a frozen cell bank containing recombinant cells are used in cultivation.
EFFECT: obtainment of recombinant proteins.
40 cl, 9 dwg, 1 ex
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