METHOD FOR PREVENTING CONTAMINATION OF SYSTEM Russian patent published in 2020 - IPC C12M1/00 C12M1/12 C12M1/26 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to biotechnological production of recombinant proteins. Method for preventing contamination of a system comprises extraction processes and associated hardware to enable separation of fluid medium flows from a system, for example a sterile process vessel, which contains a sterile process stream. Method comprises providing a system comprising a first vessel and a second vessel, wherein the first vessel comprises a liquid, and the second vessel comprises an amount of sterilizing gas, providing flow of first volume of liquid from first vessel to second vessel through volume of sterilizing gas contained in upper part of second vessel. Extraction processes enable continuous removal of fluid medium streams, in particular waste streams, a liquid containing recombinant therapeutic proteins from the system, for example, a biological production system, which provides fewer manual manipulations with the sterilized system and a lower risk of contamination of the sterilized system.

EFFECT: invention provides higher effectiveness and convenience of preventing contamination with simultaneous preservation of required quality of vascular sterility and reduced material costs.

18 cl, 2 dwg