FIELD: medicine, agriculture and veterinary medicine.
SUBSTANCE: invention relates to medicine, agriculture and veterinary medicine, namely to parasitology and can be used to detect the nerve ring in larvae of parasitic nematodes of the order Strongylida. Photographs of different depths of field of the nematode larva are obtained, which is in a lateral position in a micropreparation. The images are stacked to obtain an image that shows both internal and surface structures of the larva. On the obtained image, the head end of the larva is determined; find the excretory opening and the excretory canal going to it. A line is drawn so that it coincides with the direction of the excretory canal and crosses the longitudinal axis of symmetry of the nematode larva. From the point of the excretory opening, a line is drawn perpendicular to the longitudinal axis of symmetry of the larva; then, from the point of the excretory opening, a line is built that is symmetrical to a line drawn along the excretory canal; at the intersection of this line and the longitudinal axis of symmetry, the nematode larvae build a perpendicular to the axis of symmetry of the nematode larva, which corresponds to the position of the nerve ring.
EFFECT: method ensures the accuracy of the detection of the nerve ring due to topographic constructions.
1 cl, 5 dwg
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Authors
Dates
2023-01-10—Published
2022-04-27—Filed