METHOD FOR OBTAINING VIRUS-LIKE PARTICLES OF NOROVIRUS CONTAINING THE PROTEIN VP1 OF ENTEROVIRUS ECHOVIRUS 30, IN VITRO Russian patent published in 2023 - IPC C07K14/85 

FIELD: molecular virology.

SUBSTANCE: invention relates to the field of molecular virology, biotechnology and medicine, in particular to the production of a chimeric protein that spontaneously forms in vitro virus-like particles (VLP) containing the protein VP1 Echovirus 30 (ECHO30). The obtained virus-like norovirus particles containing the protein VP1 of the enterovirus Echovirus 30 on their surface can be used to prepare vaccines against non-polio enteroviruses. The method for obtaining virus-like particles of norovirus containing the protein VP1 Echovirus 30 (ECHO30) in vitro includes cultivation of E. coli cells transformed by chimeric protein in a suitable culture medium, isolation and purification of virus-like particles. In this case, a chimeric protein S_N-VP1_E30 is created with a size of 516 amino acids with a molecular weight of 57 kDa, with a structure in accordance with the sequence SEQ ID NO: 1 and the transformation of E. coli cells, Rosette strain 2 (DE3) with a plasmid containing a nucleotide sequence encoding the amino acid sequence SEQ ID NO: 1.

EFFECT: implementation of the invention provides for the creation of a chimeric protein SN-VP1E30 with a size of 516 amino acids with a molecular weight of 57 kDa, which is capable of spontaneously assembling into VLP presenting antigenic determinants of the protein VP1 of enterovirus E30.

1 cl, 4 dwg, 6 ex