DEFROSTING LIQUID, ITS PREPARATION METHOD AND ITS USE Russian patent published in 2023 - IPC A01N1/02 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of preservation of biological material, in particular, to defrosting of cryopreserved cells or tissues. A defrosting solution contains, per 100 ml: 0.1-50 g of biomimetic material for suppression of ice growing, water-soluble saccharide at a concentration of 0-1.0 mol/l, and the rest is a buffer. Biomimetic material for suppression of ice growing is atactic polyvinyl alcohol (PVA) having syndiotacticity of 15-65%, molecular weight of 10-500 kDa, or a combination of the specified atactic polyvinyl alcohol (PVA), amino acid, polypeptide, and/or polyamino acid. Amino acid is selected from one of or a combination of two or more of arginine, threonine, proline, lysine, histidine, glutamine, aspartic acid, glycine. Polypeptide is one or more of L-Thr-L-Arg (TR), L-Thr-L-Pro (TP), L-Arg-L-Thr (RT), L-Pro-L-Thr (PT), L-Thr-L-Arg-L-Thr (TRT), L-Thr-L-Pro-L-Thr (TPT), or L-Thr-L-Cys-L-Thr (TCT). The defrosting solution is used in a defrosting reagent, which contains defrosting solutions I-IV, wherein defrosting solutions I-IV have different compositions by quantitative content of initial components.

EFFECT: proposed defrosting solution provides effective control of ice crystal growth, as well as significant reduction in cell or tissue damage caused by uncontrolled ice crystal growth during reduction, reduction in risks of a short service life and inclusion of parasitic biological contaminants to bonds with the absence in a composition of animal serum, contributes to preservation of stability, when passaging cells or tissues, has low toxicity.

10 cl, 4 dwg, 2 tbl, 3 ex