CRYOPRESERVING SOLUTION NOT CONTAINING DMSO AND ITS PRODUCTION METHOD AND USE Russian patent published in 2022 - IPC A01N1/02 A01N1/00 C07K5/00 C07K5/68 C07K5/78 C07K5/83 

FIELD: biotechnology.

SUBSTANCE: group of inventions is described, including a cryopreserving solution not containing DMSO, a method for the production of a cryopreserving solution, a freezing balancing solution not containing DMSO, a cryopreserving reagent not containing DMSO, and the use of a cryopreserving solution and/or a freezing balancing solution for cryopreservation of biological tissues. In one of implementation options, the cryopreserving solution contains, per 100 ml, 0.01-50 g of biomimetic material for suppression of ice accumulation, 5.0-45 ml of multiatomic alcohol, water-soluble saccharide at a concentration of 0.1-1.0 mol/l, 0-30 ml of serum, and the rest is a buffer, where biomimetic material for suppression of ice accumulation is selected from atactic PVA characterized by syndiotacticity of 15-60%, molecular weight of 10-500 KDa or more, and a hydrolysis degree of more than 80%, or the specified atactic PVA and amino acid biomimetic material for suppression of ice accumulation.

EFFECT: invention expands the arsenal of means for cryopreservation.

12 cl, 10 dwg, 9 tbl, 8 ex