MONOCLONAL ANTIBODY SPECIFICALLY BINDING TO THE THIOREDOXIN-1 EPITOPE, ITS PREPARATION AND APPLICATION Russian patent published in 2023 - IPC C07K16/18 C12N15/13 C12N15/63 G01N33/543 G01N33/574 A61K39/395 C12P21/08 

FIELD: immunology.

SUBSTANCE: group of inventions relates to immunology. A monoclonal antibody specifically binding to thioredoxin-1 (Trx1) or its antigen-binding fragment was proposed, including a variable region of the light chain containing CDR1 with SEQ ID NO: 7, CDR2 with SEQ ID NO: 8, CDR3 with SEQ ID NO: 9, and a variable region of the heavy chain containing CDR1 with SEQ ID NO: 10, CDR2 with SEQ ID NO: 11, CDR3 with SEQ ID NO: 12. Nucleic acid molecules encoding light and heavy chains of a monoclonal antibody or its antigen-binding fragment, a recombinant expression vector, a host cell for obtaining a monoclonal antibody or its antigen-binding fragment, a set and methods for the diagnosis of breast cancer are also proposed.

EFFECT: monoclonal antibody has excellent binding affinity with thioredoxin-1, has high sensitivity and specificity, which significantly improves the accuracy and reliability of breast cancer diagnosis.

25 cl, 40 dwg, 25 tbl, 17 ex