METHOD FOR CULTIVATION OF CHAETOCEROS MUELLERI AND ISOCHRYSIS GALBANA UNICELLULAR MICROALGAE - LIVE FOOD FOR LARVAE OF MARINE INVERTEBRATES Russian patent published in 2023 - IPC A23K10/00 

FIELD: biotechnology.

SUBSTANCE: cultivation is carried out in repeated technological cycles for an unlimited time. Each cycle lasting 32 days consists of two stages: the stage of growing the inoculum in closed cultivators in the accumulative mode and the stage of obtaining fodder biomass in open cultivators, first in the accumulative mode, then in the quasi-continuous mode of cultivation. The date of the beginning of each subsequent cycle of growing algae is shifted relative to the previous one by 8 days. The cultivation of microalgae at all stages is carried out in a pre-prepared nutrient medium F/2 based on natural sea water, with round-the-clock illumination with a surface illumination of 14–16 klx, a temperature of 20–23°C, continuous bubbling of air through aquarium sprayers at a rate of 1.0–1.25 l/min per 1 liter of culture to maintain the pH of the medium in the range of 8.5–8.9.

EFFECT: invention makes it possible to ensure unlimited daily production of fodder biomass of Chaetoceros muelleri and Isochrysis galbana microalgae while maintaining their stable quality in all cycles.

1 cl, 2 ex