METHOD FOR CULTURING UNICELLULAR GREEN MICROALGAE DUNALIELLA SALINA FOR BIOMASS PRODUCTION Russian patent published in 2015 - IPC A01G33/00 C12N1/12 

FIELD: biotechnology.

SUBSTANCE: culture grown on the modified nutrient Trenkenshu medium by enrichment culture procedure to the density of 1.5-3 g OR·l^-1 is transformed into a quasi-continuous culture process. The following growth procedure is performed at a specific medium flow rate of approximately 0.3 days^-1 under continuous light at a superficial lighting of 80 Wt·m^-2, with continuous blow of air-gas mixture at 1 l of the mixture·min^-1·l^-1 of the culture, which contains 3 % CO₂, and a temperature of 26-28°C on the modified nutrient Trenkenshu medium. The biomass production rate has been shown at approximately 0.5 g of O.M. with 1 l of the culture a day at the relative carotinoid content in the biomass of not less than 0.9 % O.M., chlorophyll A - 2.8% O.M. and protein - 55 % O.M.

EFFECT: producing the biomass with using the quasi-continuous culture process.