FIELD: biotechnology.
SUBSTANCE: set of primers and a probe for determination of SARS-CoV-214 mutation S:Ins214EPE is described with the following composition: forward primer 214-F - SEQ ID NO: 1; reverse primer 214pure-R - SEQ ID NO: 2; fluorescent probe 214-Z2 - SEQ ID NO: 3. Synthesized oligonucleotides SEQ ID NO NO: 1-3 do not form cross-reactions with other tested samples, a selected site is amplified with 100% specificity allowing to determine presence or absence of a significant target S:Ins214EPE in the samples of biological material.
EFFECT: determination of SARS-CoV-2 mutation S:Ins214EPE.
3 cl, 1 tbl, 5 ex
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