FIELD: genetic engineering
SUBSTANCE: group of inventions relates to the field of genetic engineering and biotechnology. A method for detecting SARS-CoV-2 virus RNA, a specific oligonucleotide and a set for use in a method for detecting SARS-CoV-2 virus RNA are proposed. The method for detecting SARS-CoV-2 virus RNA uses guide RNAs represented by SEQ ID NO: 1-4 and specific oligonucleotides for preliminary amplification represented by SEQ ID NO: 5-8.
EFFECT: inventions make it possible to efficiently detect the RNA of the SARS-CoV-2 virus after carrying out specific amplification of fragments of the genome of this virus and ensures the detection of single copies of the RNA of the SARS-CoV-2 virus.
5 cl, 16 dwg, 5 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR OBTAINING A PREPARATION OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR / Cas14 AND A PREPARATION FOR DETECTING THE RNA OF THE SARS-CoV-2 VIRUS IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764024C1 |
| CRISPR-CAS14 SYSTEM FOR DETECTING SARS-CoV-2 VIRUS RNA AT ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764020C1 |
| METHOD FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2764021C1 |
| METHOD FOR OBTAINING A PREPARATION OF THE CRISPR/Cas 12 RIBONUCLEOPROTEIN COMPLEX AND PREPARATION FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764015C1 |
| CRISPR-Cas 12 SYSTEM FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764023C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
|
RU2802079C1 |
| METHOD OF OBTAINING A PREPARATION OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-CAS12 AND A PREPARATION FOR DETECTING HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) RNA IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2802781C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) RNA IN ULTRA-LOW CONCENTRATIONS IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2802783C1 |
| METHOD OF PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-CAS AND A PREPARATION FOR DETECTING HEPATITIS C VIRUS RNA OF GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2799430C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING HEPATITIS C VIRUS RNA GENOTYPES 1B AND 3A AT ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2800421C1 |
