FIELD: genetic engineering; biotechnology.
SUBSTANCE: group of inventions relates to the field of genetic engineering and biotechnology. A method for detecting SARS-CoV-2 virus RNA, a specific oligonucleotide and a kit for use in the method for detecting SARS-CoV-2 virus RNA are proposed. The SARS-CoV-2 virus RNA detection method uses guide RNAs and specific oligonucleotides for pre-amplification.
EFFECT: inventions make it possible to effectively detect the RNA of the SARS-CoV-2 virus after specific amplification of fragments of the genome of this virus and also provide detection of single copies of the SARS-CoV-2 virus RNA.
5 cl, 11 dwg, 5 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR OBTAINING A PREPARATION OF THE CRISPR/Cas 12 RIBONUCLEOPROTEIN COMPLEX AND PREPARATION FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764015C1 |
| CRISPR-Cas 12 SYSTEM FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764023C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
|
RU2802079C1 |
| METHOD OF OBTAINING A PREPARATION OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-CAS12 AND A PREPARATION FOR DETECTING HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) RNA IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2802781C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) RNA IN ULTRA-LOW CONCENTRATIONS IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2802783C1 |
| METHOD OF PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-CAS AND A PREPARATION FOR DETECTING HEPATITIS C VIRUS RNA OF GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2799430C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING HEPATITIS C VIRUS RNA GENOTYPES 1B AND 3A AT ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2800421C1 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS AT ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2782951C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
|
RU2800420C1 |
| METHOD FOR PRODUCING AN AGENT OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas12 AND AGENT FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2792891C1 |
