METHOD FOR DETERMINING THE DEGREE OF RESISTANCE OF STARCHES AND STARCH PRODUCTS Russian patent published in 2023 - IPC G01N33/10 C12Q1/40 

FIELD: food industry; biotechnology.

SUBSTANCE: method for determining the degree of resistance of starches and starch products involves sample preparation, gelatinization, fermentation-thermostation, centrifugation, separation of the filtrate, washing and dissolution of the precipitate, repeated fermentation-thermostation, determination of the carbohydrate composition of the sample on a chromatograph using high-performance liquid chromatography. In this case, a 1% solution of starch or starch product is gelatinized in a volumetric flask per 100 cm³ at 85°C for 15 minutes, the resulting paste is cooled to room temperature, 10 cm³ of a phosphate buffer solution with pH = 6.86 is added to it and the volume is adjusted to the mark with distilled water, mixed thoroughly, 4 cm³ of pancreaticα-amylase with an activity of 100 U/cm³ and 0.4 cm³ of amyloglucosidase with an activity of 300 U/cm³, this mixture is stirred and incubated at a temperature of 37°C for 1 hour. Then the hydrolysis is stopped by adding an ethanol solution, the hydrolyzate is centrifuged and the supernatant is taken to determine the carbohydrate composition on a liquid chromatograph (sample 1), and the resulting precipitate is washed and dissolved in 2 cm³ of a 2M potassium hydroxide solution, poured 8 cm³ buffer solution of sodium acetate pH=3.86 and 0.1 cm³ amyloglucosidase activity of 300 U/cm³, maintained at a temperature of 50°C for 90 minutes and the determination of the carbohydrate composition is carried out on a liquid chromatograph (sample 2). The calculation of the degree of resistance is carried out according to the formula.

EFFECT: invention provides an accurate, highly efficient and simple method for quantitative determination of the degree of resistance of starches, starch products and products containing starch.

1 cl, 1 dwg, 1 tbl, 3 ex