FIELD: biotechnology.
SUBSTANCE: invention can be used to assess in vitro specificity of molecules that have affinity for cell membrane biomarkers and are based on antibodies, antibody fragments, aptamers or alternative scaffold proteins. A method which consists in culturing cells to obtain a cell culture based on a single C6 cell line is presented. Then retroviral transduction of genes of the studied biomarker is carried out with its subsequent expression on the cell membrane, the studied biomolecule is added and the said biomolecule binds to the biomarker on the cell membrane, after which the assessment is carried out by any known method.
EFFECT: method provides a simultaneous high-precision assessment of the binding specificity of various affinity biomolecules.
1 cl
| Title | Year | Author | Number |
|---|---|---|---|
| PSCA-CAR-YT CELL LINE HAVING SURFACE EXPRESSION OF CHIMERIC ANTIGEN RECEPTORS AND EXHIBITING CYTOTOXIC ACTIVITY ON PSCA-POSITIVE HUMAN CANCER CELLS | 2018 |
|
RU2712901C1 |
| RECOMBINANT HUMAN BREAST CANCER CELL LINE BRCCH4E-134, EXPRESSING HUMAN PROSTATE-SPECIFIC MEMBRANE ANTIGEN | 2019 |
|
RU2726541C1 |
| RECOMBINANT CYTO-CAR-YT-LACT CELL LINE EXHIBITING HIGH CYTOTOXIC ACTIVITY WITH RESPECT TO PSCA-POSITIVE HUMAN CANCER CELLS | 2019 |
|
RU2724431C1 |
| ARTIFICIAL GENE MEL-TCI, CODING POLYEPITOPIC PROTEIN-IMMUNOGENE MEL-TCI, RECOMBINANT PLASMID DNA PMEL-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE MEL-TCI AND ARTIFICIAL PROTEIN-IMMUNOGENE MEL-TCI, CONTAINING CTL- AND Th-DETERMINANTS OF MELANOMA ANTIGENES RESTRUCTURED BY PLURAL ALLELS HLA OF I AND II CLASS | 2017 |
|
RU2650872C1 |
| METHOD FOR CREATING ANTI-INFECTIOUS IMMUNOLOGICAL PROTECTION AGAINST SALMONELLA TYPHIMURIUM AND LISTERIA MONOCYTOGENES USING TRANSGENESIS OF T-LYMPHOCYTES | 2018 |
|
RU2706554C1 |
| METHOD OF DETERMINING THE RISK OF MALIGNANT TRANSFORMATION OF PROSTATE CELLS | 2022 |
|
RU2806518C1 |
| HUMAN MELANOMA CELL LINE 26G WHICH SECRETES RECOMBINANT GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR | 2008 |
|
RU2395571C1 |
| HUMAN MELANOMA CELL LINE 31G WHICH SECRETES RECOMBINANT GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR | 2008 |
|
RU2395573C1 |
| COMPOSITIONS AND METHODS FOR IMMUNOTHERAPY | 2013 |
|
RU2729401C2 |
| METHOD FOR PRODUCING ANTI-TUMOR IMMUNOLOGICAL PROTECTION TO EL-4 LYMPHOMA CELLS | 2018 |
|
RU2708558C1 |
