FIELD: biotechnology; veterinary medicine.
SUBSTANCE: immunoassay test system and a method of its use are described. The test system contains a set of the following reagents: Immunosorbent, Conjugate-1, Conjugate-2, RRK-1, RRK-2, K+, K-, PR, Stop reagent and TMB-substrate solution. The invention can be used for qualitative detection of total antibodies to bovine leukemia virus (BLV) in individual or combined samples of blood serum and individual samples of milk of cattle.
EFFECT: increase of the sensitivity of detection of antibodies to BLV through the use of a “sandwich” ELISA format with biotin-streptavidin system and the detection of all classes of antibodies to 11 known BLV genotypes.
3 cl, 1 dwg, 7 tbl, 2 ex
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