FIELD: veterinary.
SUBSTANCE: obtained is strain 1H8 of hybridomal line of cells of mouse Mus. musculus - producent of monoclonal antibodies to IgG of sheep, suitable for biotechnology in elaboration of diagnostic preparations. Strain is deposited with Special Collection of re-inoculated somatic cell cultures of agricultural and commerciall sold animals by No 73. When determined by method of immuno-enzymatic analysys (IEA) antibodies titers in native culture liquid constituted in IEA 1:32-1:64, in ascitic liquid 1:640-1:5120. Monoclonal antibodies are specific to immunoglobulin IgG of sheep and do not react with immunoglobulin of cattle. When used for fixation on solid phase of glycoproteidal antigen of cattle (C) leucosis virus (in composition of complex glycoproteidal antigen- monoclonal antibodies of sheep to glycoproteidal antigen) in IEA, they ensure strength of fixation and optimal availability of antigen for antibodies in testes samples.
EFFECT: strain 1H8 can be used in production of immuno-enzymatic test-system for diagnostics of C leucosis, which will allow to increase efficiency of sanitation measures, reduce terms of enhancement of adverse in terms of leucosis cattle-breeding farms.
1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIAGNOSTICS OF CATTLE LEUCOSIS | 2008 |
|
RU2377962C1 |
| STRAIN 5A10 OF PERMANENT HYBRIDOMAL LINE OF CELLS OF MOUSE Mus. musculus - PRODUCENT OF MONOCLONAL ANTIBODIES TO IgG OF CATTLE | 2008 |
|
RU2377299C1 |
| STRAIN 8C12 OF PERMANENT INTER-SPECIES HYBRID LINE OF CELLS OF MOUSE Mus. musculus AND SHEEP Ovis aries - PRODUCENT OF MONOCLONAL ANTIBODIES TO GLYCOPROTEIDAL ANTIGEN OF VIRUS OF CATTLE LEUCOSIS | 2008 |
|
RU2377297C1 |
| STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODY RAISED TO L-CHAINS OF IMMUNOGLOBULINS | 1995 |
|
RU2093572C1 |
| STRAIN OF HYBRID CELLS MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO CANINE ADENOVIRUS HEXONE OF THE FIRST SEROTYPE (CAV-1) | 1995 |
|
RU2095411C1 |
| STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODY RAISED TO PIG JGG | 1995 |
|
RU2093574C1 |
| STRAIN OF F26 OF CONSTANT MONOCLONAL ANTIBODY LINE OF CELLS OF MUS MUSCULUS MOUSE - PRODUCER OF MONOCLONAL ANTIBODIES TO OLIGOPOLYSACCHARIDE (OPS) ANTIGEN B ABORTUS | 2014 |
|
RU2560260C1 |
| STRAIN OF HYBRID CULTIVATED CELLS OF ANIMALS MUS MUSCULUS USED FOR OBTAINING MONOCLONAL ANTIBODIES TO IGM OF CATTLE | 0 |
|
SU1560549A1 |
| STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO PIG IGM | 1995 |
|
RU2093573C1 |
| ELISA TEST SYSTEM FOR THE DETECTION OF ANTIBODIES TO BLV IN BLOOD SERUM AND MILK OF CATTLE | 2023 |
|
RU2800607C1 |
