AGENT FOR DIAGNOSTICS OF BOVINE LEUKAEMIA AND METHOD OF ITS APPLICATION Russian patent published in 2015 - IPC G01N33/48 

FIELD: veterinary medicine.

SUBSTANCE: agent for diagnostics of bovine leukaemia comprises a water soluble protein fraction of the cell line of porcine embryonic kidney, contaminated with oncornaviruses C and D, and obtained by culturing under anaerobic conditions, having a molecular weight of from 75 to 82 kD in an amount of 0.49-0.52 wt % and characterized by the presence of peaks at the wavelength of 214 nm in the ultraviolet spectral range, and additionally comprises phosphate-buffered saline. The method consists in sampling of animal blood, preparing suspension of citrated blood of 0.25-0.5% concentration, mixing the suspension with the agent for diagnostics with the ratio of the agent and the suspension of 1:4-6, maintaining the mixture at a temperature of 4-8°C for 12-15 hours, and recording the results of the reaction by the nature of the interaction of the citrated blood suspension with the agent for diagnostics, at that the presence of leukaemia is determined based on the positive reaction of the citrated blood suspension with the agent.

EFFECT: use of inventions enables to expand the range of diagnostic products, to generate new approaches to the problem of diagnostics of bovine leukaemia, to diagnose bovine leukaemia quickly and cost-effectively while maintaining efficiency of diagnostic tests.

2 cl, 2 tbl, 2 ex, 1 dwg