LIGAND-BINDING MOLECULE WITH REGULATED LIGAND-BINDING ACTIVITY Russian patent published in 2023 - IPC C07K14/52 C07K16/24 C07K19/00 C12P21/00 A61K38/19 A61K39/395 A61P35/00 A61P37/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the production of ligand-binding molecules, and can be used in medicine for the treatment of a disease associated with a target tissue. A ligand-binding molecule is proposed, where the ligand is a cytokine or chemokine, and the molecule is a polypeptide containing an antibody VH, an antibody VL and an IgG antibody constant region and having at least one urokinase (uPA), matriptase (MT-SP1) or metalloproteinase cleavage site. The cleavage site is located on the border between the VH of the antibody and the constant region of the antibody or on the border between the VL of the antibody and the constant region of the antibody, and the indicated VH and VL are connected to each other and this binding is eliminated by cleavage of the cleavage site by the protease, and the binding of the ligand by the molecule cleaved by at least one protease cleavage site is attenuated, the biological activity of the ligand is inhibited upon binding to an antibody whose VH and VL are bound to each other, and the ligand is released when the ligand-binding molecule is cleaved at the cleavage site.

EFFECT: obtaining a ligand-binding molecule that selectively activates a ligand in a target tissue.

17 cl, 31 dwg, 13 tbl, 15 ex