BINDING ANALYSIS Russian patent published in 2023 - IPC G01N21/45 G01N21/77 G01N33/68 

FIELD: biotechnology.

SUBSTANCE: method of determining the binding activity of an agent containing lymphocyte activation gene 3 (LAG-3) protein or its fragment, derivative or analogue is described. The binding of LAG-3 protein, its fragment, derivative or analogue to Class II Major Histocompatibility Complex (MHC) molecules is determined using biolayer interferometry (BLI). Moreover, this method includes determining the binding of LAG-3 protein, its fragment, derivative or analogue to Class II MHC molecules present on cells expressing Class II MHC; and LAG-3 protein, fragment, derivative or analog thereof is immobilized on the BLI probe reagent layer and Class II MHC expressing cells are in solution. A sensor for BLI is presented for determining the activity of a fragment, derivative or analogue of LAG-3 protein in binding to Class II MHC molecules, which contains a reagent layer on which the fragment, derivative or analogue of LAG-3 protein is immobilized. A kit is presented for determining the activity of LAG-3 protein or its fragment, derivative or analogue in binding to Class II MHC molecules, which includes a BLI probe having a reagent layer on which LAG-3 protein or its fragment, derivative or analogue is immobilized, as well as cells expressing Class II MHC.

EFFECT: invention makes it possible to determine the specific binding of LAG-3 derivative IMP321 to MHC class II molecules expressed on the surface of immune cells.

22 cl, 25 dwg, 17 tbl, 11 ex