ANALYSES Russian patent published in 2025 - IPC G01N33/50 C12Q1/6897 C12N5/783 

FIELD: biotechnology.

SUBSTANCE: invention relates to molecular biology. Described are methods of analysis in vitro for determining activity of a lymphocyte activation gene 3 (LAG-3). Method involves providing a plurality of effector T-cells, each effector T-cell expressing on its surface LAG-3 and a T-cell receptor (TCR) and contains a reporter gene encoding a reporter, wherein expression of said reporter is regulated by mediated LAG-3 inhibition of TCR signalling in said effector T cells. Further, the activity of said agonist is determined by the degree to which the expression of said reporter is changed in the presence of said agonist compared to expression of said reporter in the absence of said agonist, wherein said method is carried out in the absence of a natural ligand LAG-3. Also described are kits for implementing said method and use thereof.

EFFECT: property of IMP761, through LAG-3 agonism, to inhibit TCR signalling, in particular NFAT-regulated gene expression, in LAG-3 positive T-cells.

50 cl, 5 dwg, 4 tbl, 3 ex