METHOD OF DETERMINING EFFECTIVENESS OF TREATMENT OF ENDOTHELIAL DYSFUNCTION IN METABOLIC AND FUNCTIONAL DISORDERS IN EXPERIMENTAL RATS Russian patent published in 2023 - IPC G01N33/50 G01N33/68 

FIELD: medicine; pathophysiology and toxicology.

SUBSTANCE: invention is intended to determine the effectiveness of treatment of endothelial dysfunction in metabolic and functional disorders of lead intoxication in an experiment. After the end of the period of lead intoxication, rats are given daily intramuscular injection of L-arginine for 30 days at a dose of 10 mg/kg body weight. Organ samples and blood are taken from the heart, centrifuged and the MDA concentration, SOD activity, and enzymes are determined in the following erythrocytes and organs: AlAT, AsAT, GGTP and alkaline phosphatase in blood plasma and activity of Na,K-ATPase in cells of renal, myocardial and liver tissues. The content of total metabolites (NOx), total cholesterol, LDL cholesterol, HDL cholesterol and TAG is also determined in the blood plasma. Based on the declared values of activity and content of these indicators, the effectiveness of treatment of endothelial dysfunction in metabolic disorders in rats with lead intoxication is determined.

EFFECT: use of the invention makes it possible to increase the accuracy and reliability of determining the effectiveness of treatment of endothelial dysfunction, as well as to increase the level of reproducibility, adequacy, accessibility and safety for the life of the body.

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