METHOD FOR DIAGNOSING ENDOTHELIAL DYSFUNCTION IN METABOLIC AND FUNCTIONAL DISORDERS Russian patent published in 2023 - IPC G09B23/28 G01N33/48 A61B5/145 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and can be used in the diagnosis of endothelial dysfunction in metabolic and functional disorders of lead intoxication in an experiment. The method involves lead intoxication by daily administration of lead acetate to rats. In this case, the administration of lead acetate is carried out for 30 days at a dose of 5 mg/kg of body weight. After lead intoxication, samples of organs and blood from the heart are taken, centrifuged, then the concentration of malondialdehyde-MDA is determined in erythrocytes and organs, the activity of the antioxidant system-AOS, organ-specific enzymes AlAT, AsAT, GGTP and alkaline phosphatase in blood plasma and Na+, Ka+ adenosine triphosphatases in renal, myocardial and in hepatic tissues, as well as in blood plasma, the content of total metabolites of nitric oxide, adrenaline, total cholesterol-TC, low-density lipoprotein cholesterol-LDL, high-density lipoprotein cholesterol-HDL is determined, and with the activity of Na+, K+ adenosine triphosphatases in the cortical layer of the kidney 1.31±0.006 mmolrn / mg protein / hour, the activity of Na+, K+ adenosine triphosphatases in the cerebral layer of the kidney 3.35±0.016 mmolrn / mg protein / hour, the activity of Na+, K+ adenosine triphosphatases in the myocardium 0.745±0.0065 mmolrn/mg protein/hour, activity of Na+, K+ adenosine triphosphatases in the liver 0.36±0.005 mmolrn/mg protein/hour, activity of AlAT 1.98±0.016 mmol/s*l, activity of AsAT 2.69±0.03 mmol/s*l, activity of GGTP 1678.1±51.18 nmol/s*l, the activity of alkaline phosphatase is 835.43±12.64 nmol / s*l, and with an increase from the norm of TC by 2.48 times, LDL cholesterol by 3.8 times, a decrease from the norm of HDL cholesterol by 2.48 times, and with their deviation from the norm towards an increase in the concentration of MDA in erythrocytes by 1.33 times, in the cortical and cerebral substance of the kidneys from the norm by 1.25 and 1.76 times, respectively, the heart by 1.79 times, in the liver by 1.94 times, and a decrease in the level of activity of AOS from the norm by 1.6 times, the content of total metabolites of nitric oxide in 1.73 times and an increase in adrenaline from the norm by 1.17 times, the presence of endothelial dysfunction in metabolic disorders in rats with lead intoxication is judged.

EFFECT: invention allows to timely identify the endothelial dysfunction in metabolic changes, improve accuracy and reliability.

1 cl, 2 dwg, 2 tbl, 1 ex