METHOD FOR DIAGNOSING RENAL AND HEPATIC VASCULAR ENDOTHELIAL DYSFUNCTION IN TYPE 2 DIABETES MELLITUS Russian patent published in 2024 - IPC G01N33/66 G01N33/68 G01N33/70 G01N33/72 G01N33/84 G01N33/92 G01N33/493 A61B5/21 A61B5/20 

FIELD: medicine; endocrinology; pathophysiology.

SUBSTANCE: invention can be used for diagnosing renal and hepatic vascular endothelial dysfunction in type 2 diabetes mellitus. Blood glucose, mmol/l, high-density lipoprotein cholesterol (HDL), mmol/l, blood pressure (BP) are determined. Patients are taken in the morning on an empty stomach with citrate, centrifuged and additionally determined in erythrocytes and blood plasma concentration of glycated hemoglobin (HbA1c), %, hydroperoxides (HP), mmol/l, malonic dialdehyde (MDA), nmol/ml, activity of antioxidant system of superoxide dismutase (SOD), conv. units, organ-specific enzymes: alanine aminotransferase (ALT), mcmol/s*l, aspartate aminotransferase (AST), mcmol/s*l, gamma-glutamyl transpeptidase (GGTP), nmol/s*l, alkaline phosphatase (AP), nmol/s*l, in blood plasma, and determining the content of total nitrogen oxide (NOx) metabolites, mcmol, total cholesterol (TC), mmol/l, low-density lipoprotein cholesterol (LDL), mmol/l, endogenous creatinine clearance—glomerular filtration rate (GFR), ml/min/1.73 m², tubular reabsorption of water (R_H2O), %, and sodium (R_Na), %, urine excretion of sodium, %, and protein, g/24 h, daily diuresis, ml/24 h. At glucose level—14.6±0.5 mmol/l, HbA1c—10.4%±0.71 %, with ALT activity—43.01±4 mcmol/s*l, AST activity—35.17±6 mcmol/s*l, GGTP activity—165.4±17 nmol/s*l, alkaline phosphatase activity—183.3±7 nmol/s*l, and with an increase in total cholesterol from the normal level, with an increase in LDL cholesterol from the normal level, with a decrease in HDL cholesterol from the normal level, with deviation of concentration of GP in blood plasma from normal level equal to 0.97±0.09 mmol/l, with deviation of concentration of MDA in erythrocytes from normal level equal to 2.93±0.177 nmol/ml, when SOD activity decreases from the normal level equal to 3.55±0.156 conv. units, with a decrease in the content of NOx, with norm of 53.64±0.831 mcmol, with an increase in blood pressure, with a decrease in GFR from level of 126.4±8.56 ml/min/1.73 m², with decrease of R_H2O, at norm 99.2±0.16 %, at decrease of R_Na, at norm 98.85±0.165 %, at increase of Na excretion with norm, at norm 63.4±3.6 %, at increase of protein excretion with urine, at rate of 0.045±0.011 g/24 h, and if the daily diuresis increases, with norm of 1,380±115 ml/24 h, endothelial dysfunction of the renal and hepatic vessels is diagnosed in type 2 diabetes mellitus.

EFFECT: method provides the possibility of increasing the accuracy and reliability, the level of reproducibility, adequacy, accessibility and completeness of the metabolic and functional parameters assessment, characterizing this pathology of renal and hepatic vascular endothelial dysfunction in type 2 diabetes mellitus, by determining the above biochemical and functional parameters.

1 cl, 4 dwg, 2 tbl, 1 ex