METHOD OF DETERMINING ELEMENTAL COMPOSITION CONTAINED IN ERESPIRABLE FRACTION OF AEROSOLS IN HUMAN SPUTUM Russian patent published in 2023 - IPC G01N15/00 G01N33/487 

FIELD: analytical chemistry.

SUBSTANCE: invention relates to a method of determining the elemental composition contained in the respirable fraction of aerosols in human sputum. A method of determining the elemental composition contained in the respirable fraction of aerosols in human sputum involves taking samples of induced sputum from a patient, after which the sputum sample is homogenized using a dispersant that ensures the destruction of cell membranes and extraction of solid aerosol particles from the biological matrix of the analyzed sample, balancing the analyzed particles in the processed sample in intense ultrasonic waves, carring out fractional separation of respirable aerosol particles, setting instrumental parameters for defining elements, carrying out the procedure of desolvation of calibration solutions and samples in an aqueous solution of 1% nitric acid, constructing a calibration dependence I = f (C), carrying out the measurement process using the ISP-MS method with an octopole reaction cell, determining the mass concentration of elements using the calibration dependences I = f (C) in μg/dm³, calculating the mass concentration of elements in a single sample in µg/dm³, calculating the mass concentration of elements X in a single sample in µg/dm³ using the following formula:

,

where C_element is the mass concentration of the element in a diluted solution of a processed sample of induced sputum in 5 cm³, established according to the calibration characteristic, μg/dm³; C_blank is the mass concentration of the element in the blank sample, established according to the calibration characteristic, μg/dm³; V_arr is the volume of the treated sample solution making 10 cm³, cm³; V_sputum is the volume of the analyzed sample of induced sputum taken for analysis making from 1 to 3 cm³, cm³; 2.5 is a dilution factor of the processed sample;η is a dilution factor of the treated sample solution — dilution factor.

EFFECT: above invention makes it possible to determine the elemental composition of the aerosol contained in the respirable fraction of human sputum using the ORS-ISP-MS method with a given accuracy in units of quantities approved for use in the Russian Federation.

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