METHOD OF PHYCOCYANINS PURIFICATION Russian patent published in 2023 - IPC A23J1/00 

FIELD: microbiological; food industries.

SUBSTANCE: following is proposed: a method of the purification of phycocyanins obtained by fermentation of microalgae, in particular those produced by Galdieria sulphuraria. The method includes (i) the stage of enzymatic decomposition of glycogen using an enzyme having the activity of α1-4-glucosidase or polygalacturonase activity, or an enzyme mixture containing an enzyme having the activity of α1-4-glucosidase or polygalacturonase activity, and an enzyme having the activity of α1-6-glucosidases, or enzymes with activity of α 1-4-glucosidase or polygalacturonase and activity of α1-6-glucosidases, at pH below 6 and reaction temperature below 40°C and (ii) the stage of separation of phycocyanins from glycogen decomposition products. A method of obtaining phycocyanin of microbial origin has been proposed; an isolated phycocyanin obtained by the claimed method is proposed, the said isolated phycocyanin including traces of enzymes with the activity of alpha1-4-glucosidase and/or 1-6-glucosidase, and/or glucose oligomers, products of enzymatic lysis of glycogen. The following is proposed: a phycocyanin extract, including phycocyanin and glycogen, where the ratio of glycogen to phycocyanin by dry weight is less than 6, and it includes traces of enzymes with alpha1-4-glucosidase and/or 1-6-glucosidase activity and/or glucose oligomers, enzymatic lysis products glycogen; the use of isolated phycocyanin or phycocyanin extract as a food coloring, as well as a food product including isolated phycocyanin or phycocyanin extract.

EFFECT: invention makes it possible to improve methods of purifying phycocyanins extracted from biomass from a qualitative point of view by reducing the residual sugar content in the final product, in particular the residual glycogen content, while maintaining the properties of phycocyanin.

18 cl, 7 dwg, 1 tbl, 4 ex