COMPOSITIONS AND METHODS FOR LABELLING BIOLOGICAL COMPOUNDS WITH STABLE ISOTOPE Russian patent published in 2011 - IPC C12N1/00 C12N5/00 C12P13/04 

FIELD: chemistry.

SUBSTANCE: microorganisms are used to prepare a culture medium, where the microorganisms are cultured on a mineral medium which supports growth of that organism, where essentially all assimilated atoms are isotope-labelled to obtain a labelled biomass. The obtained biomass undergoes autolysis to obtain a labelled autolysate. This biomass can be further extracted with an organic solvent to obtain lipids. The delipidised biomass is hydrolysed to obtain labelled amino acids and other nutrient substances which are used together with the autolysate extracted by lipids and additional components to prepare a culture medium for growing mammal or insect cells in the culture for synthesis of biomolecules. The obtained biomolecule is a mammal membrane protein, particularly in which 20-100% hydrogen atoms in that protein are totally substituted with a ²H isotope.

EFFECT: uniform labelling with stable isotopes enables to determine the three-dimensional structure of a biomolecule such as a mammal membrane protein, for example, using NMR spectroscopy.

30 cl, 4 dwg, 8 ex