METHOD OF OBTAINING VIOLACEIN EXTRACT USING JANTHINOBACTERIUM LIVIDIUM VKM B-3705D Russian patent published in 2024 - IPC C12N1/02 

FIELD: biotechnology.

SUBSTANCE: invention is a method of producing violacein extract using Janthinobacterium lividium “ВКМ” B-3705D, relates to microbiology, in particular to biotechnology. The method involves mixing Janthinobacterium lividum “ВКМ” B-3705D powder with isopropyl alcohol at room temperature to obtain a 1% colloidal solution and stirring at 150 rpm for 3 hours. Then the resulting mixture is passed under vacuum through a filter with a pore diameter of 0.1 mcm, the filtration procedure is repeated until a discolored precipitate is obtained on the filter, and all the portions of the filtrate are combined in one container. The filtrate obtained at the previous stage is evaporated using a rotary evaporator, reducing the volume by half, and the resulting extract is purified from impurities by dissolving in distilled water in a ratio of 1:10, followed by settling until a suspension is formed. The resulting suspension is filtered through a filter with a pore diameter of 0.1 mcm, after which violacein is extracted from the filter using 99% dimethyl sulfoxide. Janthinobacterium lividum “ВКМ” B-3705D powder is obtained by growing a pure culture of Janthinobacterium lividum “ВКМ” B-3705D on an agar nutrient medium containing 3% of peptone, 2% of agar and 95% of water, for 24 hours at 25°C. The resulting culture is suspended in sterile water. A 1% inoculum is obtained by adding the resulting suspension of Janthinobacterium lividum “ВКМ” B-3705D into a liquid nutrient medium containing 1% peptone and incubating the inoculum for 2 days at a temperature of 25°C and constant stirring at a speed of 150 rpm. After which the inoculum is transferred to a container with fresh nutrient medium containing 1% peptone and cultivated for 3 days with aeration with stirring at 400 rpm at 25°C ensuring that the dissolved oxygen content is maintained at 80%. Then the resulting culture liquid is frozen at minus 40°C after which it is freeze-dried.

EFFECT: proposed method expands the range of strains suitable for use as a violacein producer.

1 cl, 2 dwg