METHOD OF PRODUCING VIOLACEIN FRACTION DURING SURFACE SOLID-PHASE CULTIVATION OF JANTHINOBACTERIUM LIVIDUM B-3705D STRAIN Russian patent published in 2024 - IPC C12N1/20 

FIELD: biotechnology.

SUBSTANCE: method for surface solid-phase cultivation of the aerobic strain Janthinobacterium lividum B-3705D refers to microbiology, particularly to biotechnology, medical and agricultural biotechnology. Invention relates to surface solid-phase cultivation of an aerobic strain of Janthinobacterium lividum B-3705D and can be used for laboratory studies of metabolites obtained under controlled conditions, for example, a pigment of bacterial origin, violacein, formed during formation of a biofilm. Method involves feeding a nutrient medium into a hollow container, rotating the container until the nutrient medium hardens on the walls of the container, sowing microorganisms, incubating until the end of growth of a colony of microorganisms. After the culture medium hardens on the walls of the container, a suspension of the strain of Janthinobacterium lividum is added and the container is continued to rotate around the axis in the horizontal plane until the suspension is distributed on the culture medium of the walls of the container. Microorganisms are incubated at a constant temperature of 25 °C and during aeration with air passed through a filter with pore size of not more than 0.22 mcm. Growth of the culture of the microorganism is controlled using data on the intensity of respiration by reducing pH of 1% NaOH solution through which carbon dioxide released during the cultivation process is passed. Biofilm is considered grown and incubation is completed when the pH value of the NaOH solution does not change. Then, substances formed during cultivation are extracted by stirring for 12 hours with an organic extractant, the obtained suspension is filtered and the filtrate is evaporated. One stripped off extract is mixed with sterile distilled water at ratio of 1:10 and allowed to separate for 12 hours. After filtration, the obtained fraction of the metabolite is extracted from the membrane filter. Fraction of the metabolite is purified, its composition is analyzed and used in further studies. Using the proposed method, a research program can be implemented, for example, a metabolite synthesized by the strain of Janthinobacterium lividum B-3705D, specifically a pigment of bacterial origin, violacein, which is synthesized during formation of a biofilm.

EFFECT: disclosed is a method of producing a violacein fraction during surface solid-phase cultivation of the Janthinobacterium lividum B-3705D strain.

1 cl, 2 dwg, 1 tbl