FIELD: biotechnology.
SUBSTANCE: following is described: an oligonucleotide primer that: (a) includes at least 18 contiguous nucleotides of a sequence that is part of, or complementary to part of a reference nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 6, or (b) includes at least 18 contiguous nucleotides of a sequence that is at least 80% identical to a sequence that is the same as or complementary to a sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 80; provided that the said oligonucleotide primer does not include a sequence selected from the group consisting of SEQ ID NO: 21, SEQ ID NO: 23. The following is disclosed: the use of at least one specified oligonucleotide primer in a method of detecting a virus that infects and is capable of causing death of lumpfish (Cyclopterus lumpus), wherein the viral genome includes a nucleic acid sequence that is at least 90% identical to any of SEQ ID NO: 1, 2, 3, 4, 5, 6, and wherein the said nucleic acid sequence contains a uracil base (U) instead of a thymine base (T). The following is presented: a method of detecting a virus that infects fish and is capable of causing death of fish, comprising the following stages: (a) contact of a nucleic acid isolated from a biological fish sample with at least one oligonucleotide primer according to claim 1 to obtain a mixture, (b) determination, whether after amplification of the mixture a) an amplification product is present, where the presence of an amplification product indicates the presence of RNA associated with the virus, and therefore the presence of the virus in the biological sample. A kit for detecting a virus in a biological fish sample is disclosed, where the kit includes an oligonucleotide primer.
EFFECT: invention expands the range of tools for detecting fish diseases.
5 cl, 12 dwg, 6 tbl, 4 ex
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