MUTANT P-HYDROXYPHENYLPYRUVATEDIOXYGENASE, NUCLEIC ACID ENCODING IT, AND THEIR USE Russian patent published in 2022 - IPC C12N9/02 C12N5/10 C12N15/53 C12N15/63 C12N15/82 

FIELD: biochemistry.

SUBSTANCE: invention relates to mutant protein of p-hydroxyphenylpyruvatedioxygenase (hereinafter – HPPD) or its biologically active fragment, which gives plants resistance or tolerance to HPPD-inhibiting herbicides. Isolated polynucleotide containing a nucleic acid sequence encoding mutant HPPD or its biologically active fragment, fused protein, a nucleic acid structure, an expression vector, and a host cell containing polynucleotide are also proposed. A method for obtainment of a plant having increased resistance or tolerance to HPPD-inhibiting herbicide compared to a plant with HPPD genes of a wild type, a plant obtained by the method, as well as a method for increasing resistance or tolerance of a plant cell, plant tissue, a plant part, or a plant to an herbicide inhibiting HPPD, compared to a plant cell, plant tissue, a plant part, or a plant with HPPD genes of a wild type, including expression of mutant HPPD, or its biologically active fragment, or the above-mentioned fused protein, are also proposed.

EFFECT: invention allows for obtainment of plants having resistance or tolerance to herbicides inhibiting HPPD, while the specified mutant HPPD saves or enhances a capability of catalyzation of conversion of para-hydroxyphenylpyruvate (HPP) into homogenizate, and it is significantly less sensitive to herbicides inhibiting HPPD, than HPPD of a wild type.

22 cl, 17 dwg, 14 tbl, 10 ex