FIELD: biotechnology.
SUBSTANCE: invention relates to a fused protein containing in the direction from the N-terminus to the C-terminus a DNA methyltransferase domain, an enzyme nuclease-deficient RNA-directed DNA endonuclease and a domain associated with Krüppel box or Krüppel box related domain, the enzyme nuclease-deficient RNA-directed DNA endonuclease and the domain of DNA methyltransferase, as well as the complex containing it. Also a nucleic acid molecule encoding the above fusion protein, as well as a vector and a cell containing it are disclosed.
EFFECT: invention is effective for silencing a target nucleic acid sequence in a cell.
60 cl, 13 dwg, 2 tbl, 12 ex
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