FIELD: biotechnology; fodder production.
SUBSTANCE: disclosed is a recombinant strain Yarrowia lipolytica — producer of encapsulated phytase Panibacillus sp. RB, characterized by amino acid sequence SEQ ID NO. 2. Strain is obtained by introducing plasmid pUV3-PhyPS into the collection strain Yarrowia lipolytica PO1f. Target plasmid insert is a phytase gene PhyPS, characterized by the nucleotide sequence SEQ ID NO. 1.
EFFECT: phytase accumulate in the cytoplasm of the recombinant producer and has high thermal stability during short-term heating at 70 °C, which makes it possible to use it in the composition of fodders obtained by the method of granulation on spray drying.
1 cl, 1 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN YARROWIA LIPOLYTICA - PRODUCER OF OBESUMBACTERIUM PROTEUS ENCAPSULATED PHYTASE | 2017 |
|
RU2664476C1 |
| RECOMBINANT YEAST STRAIN Yarrowia lipolytica - PRODUCER OF PHYTASE | 2012 |
|
RU2504579C2 |
| YEAST STRAIN Yarrowia lipolytica - LIPASE PRODUCER | 2007 |
|
RU2355754C1 |
| RECOMBINANT YEAST STRAIN YARROWIA-LIPOLYTICA LIPASE PRODUCER | 2011 |
|
RU2451075C1 |
| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
RU2810538C2 |
| RECOMBINANT STRAIN OF OGATAEA HAGLERORUM YEAST - PRODUCER OF ESCHERICHIA COLI PHYTASE | 2021 |
|
RU2785901C1 |
| STRAIN OF YEAST Candida parapsilosis - PRODUCER OF LIPASE | 2014 |
|
RU2575804C1 |
| INTEGRATIVE VECTOR Random-URA3-RPT FOR SERIAL INTRODUCTION OF MULTIPLE COPIES OF GENETIC ELEMENTS IN YEAST Yarrowia lipolytica | 2006 |
|
RU2376376C2 |
| MUTANT RECOMBINANT THERMALLY STABLE PHYTASE (VERSIONS), DNA FRAGMENT CODING SAID PHYTASE (VERSIONS) Pichia pastoris STRAIN - PRODUCER OF SAID PHYTASE (VERSIONS) | 2009 |
|
RU2472855C2 |
