METHOD FOR DETECTING TYROSINE KINASE RECEPTOR GENE TRANSLOCATIONS IN TUMOUR TISSUE SAMPLES USING RNA SEQUENCING DATA Russian patent published in 2024 - IPC C12Q1/6809 C12Q1/6827 G01N33/574 

Abstract RU 2814710 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to a method which enables to detect products of expression of chimeric genes of receptor tyrosine kinases in a wide range of samples of pathological tissues of patients on the basis of RNA expression data. Method is based on analysis of character of expression of separate genes and search of imbalance of covering of exons, which testifies to presence of structural gene rearrangement. Approach is based on analysis of transcriptomic data, which potentially enables detection of chimeric products of all genes expressed in a sample. Besides, this method can be automated, which prevents potential errors associated with manual calculation. Application of this method will make it possible to efficiently and accurately detect chimeric transcripts in all types of tumour samples, including paraffinized samples fixed in formalin, the degree of degradation of nucleic acids in which is increased.

EFFECT: invention provides higher availability of personalized medicine and efficiency of selection of anticancer therapy.

1 cl, 18 dwg, 9 ex

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RU 2 814 710 C1

Authors

Buzdin Anton Aleksandrovich

Gurianova Anastasiia Andreevna

Luppov Daniil Vitalevich

Sorokin Maksim Igorevich

Suntsova Mariia Vladimirovna

Tkachev Viktor Sergeevich

Simonov Aleksandr Mikhailovich

Dates

2024-03-04Published

2023-12-03Filed