TEST SYSTEM FOR IDENTIFICATION OF DNA OF TISSUE OF PACIFIC MACKEREL (SCOMBER JAPONICUS) IN FISH PRODUCTS, IN MEAT-AND-BONE FISH MEAL AND FODDERS USING POLYMERASE CHAIN REACTION IN REAL TIME Russian patent published in 2024 - IPC C12Q1/68 

FIELD: veterinary science; microbiology.

SUBSTANCE: described is a test system for identifying DNA of tissue of Pacific mackerel (Scomber japonicus) in fish products, in meat-and-bone fish meal and fodders. Test system includes a buffer for polymerase chain reaction, a mixture for its implementation, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for an animal genome region and for an internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample in the form of a suspension of bacteriophage T4 with concentration of 5×10³ phage particles per 1 mcl, a negative control sample representing a mixture of recombinant plasmid DNA containing a fragment of the animal genome and a fragment of the genome of bacteriophage T4 with a nucleotide sequence: T4F TACATATAAATCACGCAAAGC — forward primer; T4R TAGTATGGCTAATCTTATTGG — reverse primer; T4P CY5 ACATTGGCACTGACCGAGTTC — probe, taken in ratio 1:1. According to the invention, a genome fragment of Pacific mackerel (Scomber japonicus) tissue with the following nucleotide sequence is used for a positive control sample: Sj-F ATAGCCCTCGCCCTAACTCT — primer; Sj-R AGGGCTCATTTGTGTCCAGG — primer; Sj-Z R6G ATCAGCGGAGGGTAGGTGTA BHQ1 — probe.

EFFECT: invention extends functional capabilities, improving accuracy of identification of fish tissue species.

1 cl, 5 tbl, 1 ex