FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents a method of producing recombinant human alpha-1-antitrypsin protein (AAT), involving the following steps: a) introducing into a eukaryotic host cell a nucleic acid sequence coding a human AAT protein, and at least an additional nucleic acid sequence coding transposase, selected from the group consisting of piggyBac, Tol-2, Sleeping beauty and Leap-In; b) culturing the eukaryotic host cell under conditions which enable expression of a nucleic acid sequence coding a human AAT protein; c) selecting a eukaryotic host cell with a nucleic acid fragment containing a nucleic acid sequence coding said human AAT protein, wherein selected cells are cells obtained by clonal method, expressing recombinant human AAT protein; and d) recovering recombinant human AAT protein from clonal cells, wherein said eukaryotic host cell is a Chinese hamster ovary (CHO) cell line.
EFFECT: present invention provides recombinant alpha-1-antitrypsin with high output and bioavailability for treating AAT deficiency.
23 cl, 15 dwg, 4 tbl, 9 ex
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