METHOD FOR ASSESSING LACTOBACILLUS VIABILITY PRESERVATION UNDER EFFECT OF VARIOUS ANTISEPTIC CONCENTRATIONS Russian patent published in 2024 - IPC C12N1/20 C12Q1/04 G01N33/48 C12R1/225 

FIELD: biotechnology; microbiology; gynaecology.

SUBSTANCE: disclosed is a method for assessing the preservation of viability of lactic acid bacteria under the effect of various concentrations of an antiseptic, characterized by that strains of Lactobacillus are introduced into a test tube with a liquid nutrient medium for preparing an inoculum with density of 0.5 according to the McFarland standard, further, an antiseptic is added in an amount required to create a final test concentration; test tubes are placed in a thermostat, incubated under aerobic conditions for 48 hours at 37 °C with subsequent subculture of contents of test tubes on agar for Lactobacillus and incubation in microaerophilic conditions for 72 hours at 37 °C with subsequent assessment of preservation of viability of Lactobacillus by presence of growth of single colonies of lactic acid bacteria on solid nutrient medium for each concentration of antiseptic.

EFFECT: invention provides preservation of maximum possible number of representatives of normal microflora of vagina in prevention and treatment of inflammatory diseases in gynaecology.

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