METHOD OF DETERMINING BACTERICIDAL PROPERTIES OF SUBSTANCES Russian patent published in 2019 - IPC C12Q1/18 C12N1/20 C12R1/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and microbiology. Disclosed is a method of determining bactericidal properties of substances. Method comprises incubation of test microorganisms Escherichia coli in amount of 5×10⁵ to 5×10⁶ of viable cells per ml in a liquid nutrient medium for 4–8 hours at 36–-38 °C in the presence of test substances and in their absence. Intensity of elastic light scattering (Iod), redox potential (E) and electric conductivity (X) are measured. Total degree of activating or inhibiting (+/-) vital activity of test microorganisms by test substances is determined by formula: ε_s=(ε_Iod+0,5ε_E+0,5ε_X)/2, where ε_Iod, ε_E and ε_X is determined by formulas ε_Y=100×(ΔYt-ΔYc)/ΔYc, where ΔY=Ye-Yb – differences of values Iod, E or X, recorded in beginning (Yb) and at end (Ye) incubation of test samples in presence of test substances (ΔYt) and in their absence (ΔYc).

EFFECT: method provides objectivity of evaluating bactericidal properties of substances.

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