FIELD: biotechnology.
SUBSTANCE: invention relates to a mammalian cell line containing an endogenous dehydroorotate dehydrogenase (DHODH) gene which is partially or completely inactivated and an expression vector suitable for producing a recombinant protein, where the expression vector comprises a sequence coding DHODH, as well as an expression system comprising a mammalian cell line comprising an endogenous dehydroorotate dehydrogenase (DHODH) gene which is partially or completely inactivated, and an expression vector containing a nucleotide sequence coding DHODH of a mammal, and at least one expression cassette. Also disclosed is a kit containing said cell line or expression system, and a culture medium without uridine.
EFFECT: invention is effective for production of recombinant protein in vitro.
17 cl, 8 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEM FOR RECOMBINANT EXPRESSION OF A PRODUCT OF INTEREST | 2014 |
|
RU2720525C2 |
| VACCINE BASED ON AAV5 FOR INDUCTION OF SPECIFIC IMMUNITY TO SARS-CoV-2 VIRUS AND/OR PREVENTION OF CORONAVIRUS INFECTION CAUSED BY SARS-CoV-2 | 2020 |
|
RU2783313C1 |
| VACCINE BASED ON AAV5 FOR THE INDUCTION OF SPECIFIC IMMUNITY TO THE SARS-CoV-2 VIRUS AND/OR THE PREVENTION OF CORONAVIRUS INFECTION CAUSED BY SARS-CoV-2 | 2021 |
|
RU2761879C1 |
| AAV5-BASED VACCINE FOR INDUCTION OF SPECIFIC IMMUNITY TO SARS-COV-2 VIRUS AND/OR PREVENTION OF CORONAVIRUS INFECTION CAUSED BY SARS-COV-2 | 2020 |
|
RU2760301C1 |
| MODULATION OF REP PROTEIN ACTIVITY WHEN PRODUCING CLOSED-END DNA (ceDNA) | 2020 |
|
RU2812850C2 |
| MOLECULES BINDING WITH CD38 AND PD-L1 | 2017 |
|
RU2764201C2 |
| COMPOSITIONS AND METHODS FOR DIAGNOSTICS AND TREATMENT OF TUMOUR | 2010 |
|
RU2563359C2 |
| CODON-OPTIMIZED NUCLEIC ACID WHICH ENCODES SMN1 PROTEIN, AND USE THEREOF | 2020 |
|
RU2742837C1 |
| BISPECIFIC ANTIBODIES BINDING TO TFR | 2019 |
|
RU2810756C2 |
| ANTI-ORAI1 ANTIBODY | 2015 |
|
RU2724742C2 |
