METHOD OF PRODUCING PROTEIN-PHOSPHATE COMPLEX FROM BEANS, HAVING INHIBITORY ACTIVITY ON α-AMYLASES Russian patent published in 2024 - IPC A61K36/48 C07K1/02 C07K1/14 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely to a method of producing a protein-phosphate complex – inhibitors α-amylase from leguminous crops. Method of producing protein-phosphate complex of beans, having inhibitory activity on α-amylase, including milling beans in a mill to flour state, sieving through a sieve with a hole diameter of 1 mm, extraction of protein substances in an ultrasonic bath with parameters of 95 V, 50/60 Hz using a phosphate buffer solution with pH 8 for 15 minutes at a ratio of bean flour : buffer solution of 1:15, centrifugation at 4,000 rpm at temperature of 5 °C for 5 minutes, vacuum filtration, precipitation of protein-phosphate complex with cooled acetone in ratio 1:2, secondary vacuum filtration, washing with cooled diethyl ether in amount of 7.5 ml per 1 g of raw material, drying of protein-phosphate complex.

EFFECT: method described above maximizes output of the end product – protein-phosphate complex and preservation of its biological activity.

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