TEST SYSTEM FOR IDENTIFICATION, EVALUATION OF GENETIC POLYMORPHISM AND GENETIC CERTIFICATION OF VARIETIES AND LINES OF TIMOTHY PHLEUM PRATENSE LINNAEUS Russian patent published in 2024 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: disclosed is a test system based on SSR-PCR and comparing the size of the obtained amplicons of SSR loci by polyacrylamide gel electrophoresis for assessing genetic diversity, identification, as well as certification of varieties and lines of fodder crop of timothy Phleum pratense L. Test system consists of two PCR mixes: 1 and 2. For genetic analysis of timothy lines and varieties, it is necessary to isolate DNA from their leaves and use 1 mcl of DNA extract for two PCR, the first one using PCR mix 1 and the second one using PCR mix 2. Further, the amplicons obtained in two reactions are analyzed in two separate gel electrophoresis in 10% polyacrylamide gel. Based on the results of two gel electrophoresis, conclusions are made on genetic diversity and relationship of lines and varieties of timothy. Results of two electrophoresis can be combined and converted into digital format 1/0, which can serve as a genetic passport of the analyzed variety.

EFFECT: disclosed is a test system for identification, evaluation of genetic polymorphism and genetic certification of varieties and lines of timothy Phleum pratense L.

1 cl, 2 dwg