FIELD: agriculture, in particular determination of plant material genotype and sunflower breeding.
SUBSTANCE: claimed method includes isolation of DNA from each sprout in seed group of sunflower plant followed by amplification thereof using PCR. Amplification is carried out on HA 432, HA 514, HA 1442, and ORS 5 microsatellite locuses by using primers corresponding to flanking regions of such locuses. Amplification products are separated by electrophoresis in colored, e.g. agarose gel. Visual comparison of electrephoresis spectra, e.g., in ultraviolet beam makes in possible to evaluate inbred line uniformity and hybridism level in tested sample based on number of single-type spectra.
EFFECT: method of increased accuracy.
5 cl, 3 dwg, 2 ex
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