FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to an expression plasmid DNA vector pET15b-p26AEI with size of 6350 bp. Said vector is intended for production of recombinant protein p26 of equine infectious anaemia virus (INAN) in a prokaryotic system and includes: an inducible Lac promoter; ampicillin resistance sequence; sequence of a fragment of a gag gene coding a basic capsid protein p26, with size of 717 bp; six polyhistidine sequences (6×His-tag) at the C-terminus of the protein; pUC ori – plasmid replication start site; bla- gene β-lactamase for selection of target clones in prokaryotic cells.
EFFECT: present invention provides high level of target gene expression and synthesis (reproduction) of recombinant protein p26.
1 cl, 10 dwg, 1 tbl, 5 ex
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