FIELD: biotechnologies.
SUBSTANCE: created is the plasmid genetic construct pET21a_SKP providing the expression of a single-domain antibody against SARS-CoV-2 in the prokaryotic system E. coli, having size of 5917 bp and the nucleotide sequence SEQ ID NO: 1. Recombinant SKP protein is obtained, which is expressed in the E. coli prokaryotic system by the plasmid genetic construct pET21a_SKP at cl. 1, having broadly neutralizing properties of a single-domain nanoantibody against SARS-CoV-2, having molecular weight of 23 kDa and amino acid sequence SEQ ID NO: 2.
EFFECT: extended range of single-domain nanoantibodies for SARS-CoV-2 virus diagnostics, as well as production of broadly neutralizing antibodies with high and stable expression yield.
2 cl, 5 dwg, 1 tbl, 5 ex
| Title |
Year |
Author |
Number |
| PLASMID GENETIC CONSTRUCT pET21_AB_CoV-2_1.3, WHICH ENSURES THE EXPRESSION OF RECOMBINANT PROTEIN AB_CoV-2_1.3 IN THE PROKARYOTIC SYSTEM OF E. COLI, AND RECOMBINANT PROTEIN AB_CoV-2_1.3, WHICH HAS THE PROPERTIES OF A SINGLE-DOMAIN NANOANTIBODY AGAINST SARS-CoV-2 |
2022 |
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- Merkuleva Yuliya Aleksandrovna
- Nesmeyanova Valentina Sergeevna
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Ilichev Aleksandr Alekseevich
|
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2023 |
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|
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2021 |
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|
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2021 |
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|
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2020 |
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2023 |
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2023 |
- Nesmeyanova Valentina Sergeevna
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|
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2023 |
- Aripov Vazirbek Salakhiddinovich
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- Ilichev Aleksandr Alekseevich
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|
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| ARTIFICIAL GENE STBL_RBD_TRM_SC2, CODING A BICISTRONIC STRUCTURE FORMED BY THE SARS-COV-2 CORONAVIRUS GLYCOPROTEIN S RECEPTOR-BINDING DOMAIN SEQUENCES, TRANSMEMBRANE REGION, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_TRM_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_TRM_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
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- Tishin Anton Evgenevich
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|
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2023 |
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