FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology and molecular biology. Described is recombinant plasmid DNA coding microRNA hsa-miR-145-5p, for diagnosing cardiovascular diseases and determining the risk of their development, wherein the recombinant plasmid DNA comprises an oligonucleotide insert with a sequence according to SEQ ID NO: 1 coding microRNA hsa-miR-145-5p, flanked by Eco47I restriction site sequences, and wherein the recombinant plasmid DNA has a sequence according to SEQ ID NO: 4 and an arrangement of elements as shown in Fig. 1. Also described is the use of recombinant plasmid DNA coding microRNA hsa-miR-145-5p, according to cl. 1 as a positive control for real-time PCR for the purpose of diagnosing cardiovascular diseases and determining the risk of their development.
EFFECT: wider range of means of diagnosing cardiovascular diseases and determining the risk of their development.
2 cl, 3 dwg, 1 tbl, 3 ex
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