FIELD: biotechnology.
SUBSTANCE: disclosed is a serum-free nutrient medium for the cultivation of transplantable animal cell cultures and the accumulation of mammalian viruses, containing, as a source of nutrients and growth factors, 0.25% mink muscle tissue hydrolyzate produced by "Bioprogress" LLC with a weight fraction of amine nitrogen content of not less than 6.3% in a sterile balanced salt solution of hanks, with pH of the medium, brought to value of 7.3±0.1 with the help of sodium hydroxide solution in concentration of 0.1 mol/l, with the antibiotic penicillin-streptomycin in concentration of 60 units/ml.
EFFECT: invention provides wider range of serum-free nutrient media and increased confluence of mammalian cells when cultured on said medium.
1 cl, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| NUTRITION MEDIUM FOR CULTIVATION OF TRANSPLANT CELL CULTURES | 2022 |
|
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| METHOD OF STABLE CELL CULTURES MANUFACTURE | 2008 |
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| LINE OF RE-GRAFTED SPLEEN CELLS OF ADULT GREEN MARMOSET 455 USED FOR CULTURING VIRUSES | 0 |
|
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| BHK-21/13-02-TRANSPLANTABLE MONOLAYER-SUSPENSION SUBLINE OF NEWBORN SYRIAN HAMSTER KIDNEY CELLS, INTENDED FOR FMD VIRUS AND RABIES VIRUS REPRODUCTION | 2005 |
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RU2614074C1 |
| STRAIN OF CULTIVATABLE CELLS OF MINIHOG USED AS TEST SYSTEM FOR REPRODUCTION AND STUDIES OF INFLUENZA VIRUS | 0 |
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SU1430403A1 |
