FIELD: biotechnology.
SUBSTANCE: invention relates to suspension culturing of BHK-21 cells transplanted crops. BHK-21/13-02 - adapted line to suspension method of cultivation using a nutrient medium for suspension cultivation based on hydrolysates: muscle and lactalbumin. BHK-21/13-02 cell culture is intended for industrial reproduction of FMD virus type A, O, C, Asia, rabies virus of "Shchelkovo-51" strain used for the manufacture of antiviral vaccines against FMD and rabies. BHK-21/13-02 cells are sensitive to the foot and mouth disease virus, accumulating in an amount of 7.01 g TCD50/ml, at immunogenic component content up to 1 mlg/ml and are sensitive to rabies virus, providing the infectivity titer of 6.5LD 50/ml.
EFFECT: increased sensitivity of cells.
| Title | Year | Author | Number |
|---|---|---|---|
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|
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