METHOD FOR DETERMINING ERYTHROCYTE DEFORMABILITY Russian patent published in 2025 - IPC G01N15/01 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, particularly to laboratory methods of erythrocyte analysis, and can be used to determine erythrocyte deformability and monitoring this characteristic in order to obtain diagnostic information on the patient's condition. Method for determining erythrocyte deformability involves collecting a sample of whole blood, or donor blood, or erythrocyte-containing components of donor blood. Mixing the selected sample with a physiological solution in a volume ratio of 1:1000 to obtain a suspension containing at least 25 cells in the field of view of the microscope, which is placed in an electrochemical microcell equipped with an optically transparent working electrode, an auxiliary electrode and a reference electrode. Suspension containing erythrocytes is then exposed to direct current in a potentiodynamic mode at rate of 10 mV/s from the value of the current-free potential corresponding to the potential of the open circuit to minus 600 mV relative to the reference electrode with video recording of the change in erythrocyte morphology. According to the results of comparing the erythrocyte images obtained at the initial moment of time at the current-free potential and when the potential value reaches minus 600 mV, determining the number of discocytes, echinocytes I, echinocytes II, echinocytes III, spheroechinocytes I, spheroechinocytes II and erythrocytes with a destroyed membrane with subsequent determination of the deformability index (DI), %, taking into account the portion of pathological erythrocyte cells (PC). When obtaining values DI ≥ 80% and PC not more than 0.0003 – conclude on normal state of erythrocytes, when obtaining value of 60% ≤ DI < 80% and PC not more than 0.008 – on subcompensated state of erythrocytes, when obtaining a value of DI < 60% and PC ≥ 0.008 – on pathological state of erythrocytes.

EFFECT: high sensitivity of the method.

1 cl, 3 dwg, 3 tbl